杉木人工林采伐迹地更新群落结构特征的研究

从杉木人工林科学经营和自然资源管理保护出发，根据定位研究和群落样地调查结果，分析和比较了湖南杉木中心产区会同杉木人工林不同采伐方式迹地上更新群落的组成结构和数量特征．结果表明：迹地群落中共有维管植物85科164属254种，其中乔木、灌木、藤本和草本分别为53种、85种、39种和77种；皆伐和间伐后物种多样性指数高；皆伐8a后迹地群落与皆伐2a后群落相似性较小，而与间伐8a后群落相似性较大；杉木人工林采伐迹地种类成分变更快．提出了合理经营和保护杉木林生态系统多样性和稳定性的措施．     

Simple method for synthesizing phenolicβ-O-4 dilignols

A modified synthetic method for phenolic-O-4 lignin substructure model dimers was developed involving protection of the phenolic hydroxyl group of acetophenons with benzoyl chloride, bromination with 4-dimethylaminopyridiniumbromide perbromide, condensation with phenols in the presence of 18-crown-6-ether, condensation with paraformaldehyde, reduction with NaBH₄ and debenzoylation. This method results in shorter reaction times and increasing yields without the application of strict anhydrous and drastic conditions or chloric solvents. This alternative route could be applied to the-O-4 dilignol syntheses of four combinations of guaiacyl and syringyl derivatives.     

四种混交林类型对杨扇舟蛾控制作用的研究

试验表明 :块状混交模式是控制杨扇舟蛾的最佳混交模式 ;在块状混交模式下 ,杨×槐、杨×桐两种混交类型为最佳混交类型 ;在块状混交模式的 3种混交比例中 ,杨×槐、杨×桐两种混交类型对杨扇舟蛾的控制作用差异不显著     

饲用酸化剂对1～63日龄黄羽肉鸡生长性能的影响

本试验旨在评估饲用酸化剂对1~63日龄黄羽肉鸡生长性能、肠道损伤和盲肠微生物菌群变化的影响,同时探讨壳寡糖对饲用酸化剂的增效作用。试验采用单因素完全随机区组设计,设对照组(Con.)、饲用酸化剂(Trt.1)和饲用酸化剂+壳寡糖(Trt.2)共3个处理。每个处理5个重复,每个重复50羽。饲养周期为63天。试验结果表明,从零日龄开始持续在黄羽肉鸡日粮中添加饲用酸化剂、饲用酸化剂+壳寡糖后,对1~21d和22~42d阶段黄羽肉鸡生长性能的影响未达显著(P0.05)水平;但在43~63d阶段时,与对照组相比,添加饲用酸化剂、饲用酸化剂+壳寡糖组的耗料增重比分别降低0.11和0.14,具有极大的生产意义;且饲用酸化剂添加组盲肠大肠杆菌/乳酸杆菌值显著(P0.05)低于对照组,饲用酸化剂+壳寡糖添加组盲肠大肠杆菌/乳酸杆菌值显著(P0.05)低于对照组和饲用酸化剂添加组。说明饲用酸化剂可通过维持肠道健康,促进养分消化吸收,从而提高饲料利用率;而壳寡糖在平衡肠道菌群、维持肠道健康方面对饲用酸化剂有增效作用。     

Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation

LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.     

湖北省三峡地区天然林生态效益评估

本文采用涵养水源、保育土壤、固碳释氧、积累营养物质、净化大气环境和生物多样性保护等9个功能指标,对湖北省三峡地区天然林生态系统服务功能价值进行评估。结果表明:湖北省三峡地区天然林生态系统服务价值为336.66亿元·年-1。其中,固碳释氧、涵养水源和生物多样性保护3项功能价值量最大,分别占该地区天然林生态系统服务总价值的34.94%,30.45%和29.00%;阔叶林生态系统服务功能价值量最大,占该地区天然林生态系统服务总价值的37.54%。固碳释氧、涵养水源和生物多样性保护等功能都发挥着重要的作用,阔叶林在湖北省三峡地区天然林生态系统中具有突出的功能。     

哀牢山保护区景东片区中山湿性常绿阔叶林特点研究

哀牢山位于云南省中部,保护区分布在哀牢山的中上部,其中景东片区面积为11 812hm2,位于哀牢山西坡北段。此次我们在哀牢山保护区主要保护对象之一的中山湿性常绿阔叶林中设置了样地,开展详细调查,并对调查数据进行了计算、统计、分析。通过各个样本乔木层、灌木层、草本层的H’值比较,发现乔木层﹥灌木层﹥草本层,表明乔木层的信息量大于灌木层,而草本层是该结构中相对较低的。     

Identification of ‘Candidatus phytoplasma ziziphi’ associated with persimmon (Diospyros kaki Thunb.) fasciation in China

Persimmon (Diospyros kaki Thunb.) trees, with fasciation symptoms (PF), were observed in an orchard located in suburban Tai'an, Shandong Province, China. A specific fragment of the phytoplasma 16S rRNA gene, approximately 1.2 kb in length, was amplified from two symptomatic plants via nested polymerase chain reaction, while no fragment was obtained from healthy controls. The two samples (PF1 and PF2) resulted with 99.5% nucleotide sequence identity. Phylogenetic and restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences revealed that PF1 was a member of ribosomal subgroup B of the elm yellows group (16SrV), and PF2 may represent a novel subgroup within the 16SrV group, designed as 16SrV‐I. This is the first report of phytoplasmas of the 16SrV group associated with persimmon fasciation disease.     

Localization of putative pituitary stem/progenitor cells in female dairy cattle

Research on sex-determining region Y-box 2 (SOX2)-positive pituitary stem/progenitor cells, as a source of hormone-producing cells, is progressing rapidly in rodents. However, the stem/progenitor cells supplying hormone-producing cells that are essential for growth, reproduction, and lactation in bovines have not yet been identified. In this study, we characterized SOX2-positive cells in the pituitary gland of dairy cattle (Holstein heifers) after sexual maturity. Immunofluorescence analysis revealed that the localization pattern of SOX2-positive cells in the dairy cattle pituitary gland was similar to that observed in the rodent pituitary gland; the marginal cell layer (MCL), dense cell clusters, and single cells scattered in the parenchyma of the anterior lobe. Furthermore, most of the SOX2-positive cells were positive for the pituitary stem/progenitor cell niche markers E-cadherin and cytokeratin 8+18, which have been reported in rodents. In addition, in the MCL of the anterior lobe, there was a subpopulation of SOX2-positive cells positive for paired-related homeobox 1 and 2, whereas negative for S100β. Moreover, in the parenchyma of the anterior lobe, co-localization of SOX2 and pituitary hormones was infrequent. In summary, this study reveals the localization of putative pituitary stem/progenitor cells positive for SOX2 in dairy cattle. These results provide valuable information to support further investigation of cell supply in the dairy cattle pituitary gland.     

Status of Biological Studies and Aquaculture Development of the Mud Crab, <Emphasis Type="Italic">Scylla serrata</Emphasis>, in China: An Experimental Ecological Studies

This is an initial paper in a series of overviews of biological research and aquaculture development of the mud crab, Scylla serrata, in China. Results of experimental ecological studies on mud crabs are reported here. As a result of these experimental studies, results that are important for mud crab culture were also discovered and these include, methods to condition and manage broodstock, determination of ecological conditions that are suitable for embryonic development, and the influence of temperature, salinity, diet and larval density on development and survival of larvae. Results of this work will be useful in establishing a good method for artificial mass culture of larvae.